To determine whether spironolactone induces a degenerative phenotype in a fly model of SBMA, we determined the viability of transgenic flies that were reared on food containing increasing concentrations of spironolactone (Sigma Aldrich) or 1% ethanol vehicle. Virgin female homozygous Elav > Gal4 flies were mated to male transgenic flies carrying the UAS-hAR52Q transgene and Cyo-GFP second chromosome balancer to produce either Elav > AR52Q (SBMA fly model) or Elav;Cyo-GFP (control) progeny [6 (link)]. Upon the presence of pupae within the mating vial containing spironolactone, parental flies were removed and emerging adult progeny were scored for the presence or absence of the Cyo-GFP phenotypic marker over the course of 10 days.
Protocol detail
Spironolactone Effects on SBMA Fly Model
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Adult progeny
Chromosome
Ethanol
Female
Flies
Food
Homozygous
Male
Parental
Phenotypic
Pupae
Sbma
Spironolactone
Transgene
Transgenic
Corresponding Organization : National Institute of Neurological Disorders and Stroke
Other organizations : St. Jude Children's Research Hospital, Howard Hughes Medical Institute, University College London, National Hospital for Neurology and Neurosurgery
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Variable analysis
independent variables
- Concentration of spironolactone (Sigma Aldrich)
- Viability of transgenic flies (presence or absence of Cyo-GFP phenotypic marker)
- 1% ethanol vehicle
- Positive control: Elav;Cyo-GFP (control) progeny
- Negative control: Not explicitly mentioned
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