The absorbance of the putative Taxol from the most potent fungal isolate was measured by UV–Vis spectrophotometry at wavelength range 200–400 nm. The concentration and purity of the extracted sample were assessed, compared to the authentic Taxol, with methanol for zeroing the spectrophotometer [16 (link)]. The Infra-Red (FT-IR) spectra of the sample were determined using a JASCO, FTIR 6100 Spectrophotometer, sample was pulverized in KBr pellets, and the spectra were recorded from 4000 to 500 cm−1.
The identity of the purified Taxol was resolved by the LC–MS/MS with a Thermo Scientific LCQ Deca mass spectrometer and Hypersil Gold aQ (C18 column) equipped with a positive ion mode electrospray source. The gradient elution mobile phase system of solution A (0.1% formic acid) and B (acetonitrile in 0.1% formic acid), was used with at 0.2 mL/min for 40 min, with a mobile phase B gradient ranging from 2 to 98% [16 (link), 17 (link), 21 (link)]. The chemical features of the committed signals were assessed by analyzing their fragmentation pattern with the NIST mass spectral library.
The identity of the purified Taxol was resolved by the LC–MS/MS with a Thermo Scientific LCQ Deca mass spectrometer and Hypersil Gold aQ (C18 column) equipped with a positive ion mode electrospray source. The gradient elution mobile phase system of solution A (0.1% formic acid) and B (acetonitrile in 0.1% formic acid), was used with at 0.2 mL/min for 40 min, with a mobile phase B gradient ranging from 2 to 98% [16 (link), 17 (link), 21 (link)]. The chemical features of the committed signals were assessed by analyzing their fragmentation pattern with the NIST mass spectral library.
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