Rabbit muscle actin, labeled actins, profilin, mouse cofilin-1, capping protein, and biotin-gelsolin were prepared as described in ref. 16 (link). ADP-actin was prepared as described in ref. 34 (link). Briefly, 30 µM ATP-G-actin solution containing 0.3 mM glucose and 1 unit/ml of hexokinase (Sigma) was dialyzed against nucleotide exchange buffer (5 mM Tris-HCl, 0.1 mM MgCl2, 0.05 mM EGTA, 0.2 mM ADP, 1 mM DTT, pH 8.0) for 4 h at 4 °C. All experiments were performed using rabbit muscle α-actin.
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