Isolation of Human Saphenous Vein Endothelial Cells

Patients undergoing aortocoronary artery bypass surgery were recruited from the Department of Cardiac Surgery at Leeds Teaching Hospitals. Primary saphenous vein (SV) endothelial cells (SVECs) were isolated from segments of human SV, obtained as previously described (18 (link)). Ethical approval was granted by the local Research Ethics Committee (Ref. No. CA01/040). SVECs were grown in EBM-2 growth medium supplemented with an EGM-2 bullet kit (Lonza) and used up to passage 3.

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Maqbool A., Watt N.T., Haywood N., Viswambharan H., Skromna A., Makava N., Visnagri A., Shawer H.M., Bridge K., Muminov S.K., Griffin K., Beech D.J., Wheatcroft S.B., Porter K.E., Simmons K.J., Sukumar P., Shah A.M., Cubbon R.M., Kearney M.T, & Yuldasheva N.Y. (2020). Divergent effects of genetic and pharmacological inhibition of Nox2 NADPH oxidase on insulin resistance-related vascular damage. American Journal of Physiology - Cell Physiology, 319(1), C64-C74.

Publication 2020

EBM-2 growth medium EGM-2 bullet kit

Aortocoronary bypass Artery Cardiac surgery Endothelial cells Growth medium Human Local ethics committee Patients Saphenous vein Surgery

Corresponding Organization : University of Leeds

Other organizations : Tashkent Pediatric Medical Institute, British Heart Foundation, King's College London

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Variable analysis

independent variables

Patients undergoing aortocoronary artery bypass surgery

dependent variables

Primary saphenous vein (SV) endothelial cells (SVECs) isolated from segments of human SV

control variables

SVECs were grown in EBM-2 growth medium supplemented with an EGM-2 bullet kit (Lonza)
SVECs were used up to passage 3

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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