WT hermaphrodite Bristol worms (N2) and GFP-tagged myosin worms81 (link) were treated with trigonelline chloride (catalogue no. T5509, Sigma-Aldrich) and NR chloride from day 1 of adulthood. Nematodes were cultured at 20 °C on nematode growth medium (NGM) agar plates seeded with Escherichia coli strain OP50. Trigonelline and the other precursors were added to the NGM medium at a final concentration of 1 mM just before pouring the plates, unless otherwise stated. An enzymatic method adapted from Dall et al.71 (link) was used to measure NAD+ content in worms. Fifty to 100 worms were collected per biological replicate and NAD+ levels were normalized on the protein content. The bacterial feeding RNAi experiments were carried out as described82 (link). RNAi was performed using the clones sir-2.1 (R11A8.4) and nprt-1 (Y54G2A.17) from Geneservice.
Other organizations :
Nestlé (Switzerland), University of Toyama, University of Melbourne, USA Mitchell Cancer Institute, University of South Alabama, University of Southampton, Novo Nordisk Foundation, University of Copenhagen, Tehran University of Medical Sciences, Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, National University Hospital, National University of Singapore, École Polytechnique Fédérale de Lausanne
Nematode type (WT hermaphrodite Bristol worms (N2) and GFP-tagged myosin worms)
Culture conditions (20 °C on NGM agar plates seeded with Escherichia coli strain OP50)
Concentration of trigonelline and other precursors (1 mM in NGM medium)
positive controls
Not specified
negative controls
Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
