Isolation of Liver Mitochondria for Analysis

Liver mitochondria were isolated from PhAM B6;129S‐Gt(ROSA)26Sor^{tm1.1(CAG‐COX8A/Dendra2)Dcc}/J or C57BL/6 mice (Jackson Labs) using the protocol modified from Preble et al.²² Briefly, approximately 200 mg of liver was homogenized, filtered and centrifuged, and the mitochondria pellet was suspended in PBS for MTT, or mitochondrial respiration buffer, MiR05²⁸ for respirometric analysis.

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Alway S.E., Paez H.G., Pitzer C.R., Ferrandi P.J., Khan M.M., Mohamed J.S., Carson J.A, & Deschenes M.R. (2023). Mitochondria transplant therapy improves regeneration and restoration of injured skeletal muscle. Journal of Cachexia, Sarcopenia and Muscle, 14(1), 493-507.

Publication 2023

C57BL/6 mice

Buffer C57bl mice Liver Liver mitochondria Mitochondria Mitochondrial Respiration Rosa

Corresponding Organization : University of Tennessee Health Science Center

Other organizations : William & Mary, Williams (United States)

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Variable analysis

independent variables

Mouse genotype (PhAM B6;129S‐Gt(ROSA)26Sor^{tm1.1(CAG‐COX8A/Dendra2)Dcc}/J or C57BL/6)

dependent variables

Mitochondrial function (MTT and mitochondrial respiration)

control variables

Liver tissue mass (approximately 200 mg)
Isolation protocol (modified from Preble et al.)
Buffers used (PBS for MTT, MiR05 for respirometric analysis)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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