Plasma Glucose, Insulin, and Estrogen Levels in Tumor-Bearing Animals

Plasma glucose and insulin levels were measured from samples collected at sacrifice from non-fasting tumor-bearing animals, as previously described [5 (link)]. Plasma glucose levels were determined by OneTouch UltraMini (LifeScan, Inc., Milpitas, CA, USA) and the insulin levels were determined with the rat/mouse insulin ELISA kit (EMD Millipore), according to the manufacturer’s instructions. Plasma estrogen was measured with the 17-beta-estradiol ELISA Kit (Abcam).

Free full text: Click here

Kariagina A., Morozova E., Hoshyar R., Aupperlee M.D., Borin M.A., Haslam S.Z, & Schwartz R.C. (2020). Benzophenone-3 promotion of mammary tumorigenesis is diet-dependent. Oncotarget, 11(48), 4465-4478.

Publication 2020

OneTouch UltraMini rat/mouse insulin ELISA kit 17-beta-estradiol ELISA Kit

17 beta estradiol Animals Elisa Estrogen Glucose Insulin Mouse Plasma Tumor

Corresponding Organization :

Other organizations : Michigan State University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma glucose levels
Plasma insulin levels
Plasma estrogen (17-beta-estradiol) levels

control variables

Non-fasting tumor-bearing animals

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!