Cell Labeling and Imaging in Embryos

Embryos were dissected at 5–8S and individual embryos labeled with DiI (CM-DiI, Molecular Probes) as described (3 (link)). Bright field and fluorescent images were produced using an ANDOR Zyla camera and NIS Elements BR software to record the position of the labeled cells and cultured as documented above.

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Palaria A., Angelo J.R., Guertin T., Mager J, & Tremblay K.D. (2018). Patterning of the hepato-pancreatobiliary boundary by BMP reveals heterogeneity within the murine liver bud. Hepatology (Baltimore, Md.), 68(1), 274-288.

Publication 2018

CM-DiI NIS Elements BR

Cm dii Embryos Molecular probes

Corresponding Organization : University of Massachusetts Amherst

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Variable analysis

independent variables

Embryo dissection stage (5-8S)

dependent variables

Position of labeled cells

control variables

Labeling of individual embryos with DiI (CM-DiI, Molecular Probes)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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