Quantifying SARS-CoV-2 Antibody Neutralization
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Corresponding Organization : University of Toronto
Other organizations : Maple Leaf Medical Clinic, McGill University, Centre for Health Evaluation and Outcome Sciences, HIV Legal Network, York University, Lunenfeld-Tanenbaum Research Institute, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Cornell University, University of British Columbia, Marymount University, St. Michael's Hospital
Variable analysis
- Sera dilutions (1:10 or 1:40)
- Relative ratios (RR) of ACE2 displacement
- Neutralization threshold
- Maxisorp (ThermoFisher Scientific) assay plates
- RBD (supplied by NRC) coated on plates
- BSA in PBS-T used for blocking
- Biotinylated ACE2 (supplied by Jim Rini, University of Toronto)
- Streptavidin-Peroxidase Polymer Ultrasensitive (Sigma-Aldrich)
- ELISA Pico Chemiluminescent Substrate
- EnVision reader
- Sample-free blanks on the same plate for normalization
- WHO International Standard 20/136 used as a calibrant
- Not explicitly mentioned
- Sample-free blanks on the same plate
Annotations
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