Valproic Acid Regulates Epigenetics

Valproic acid sodium salt (VPA) was purchased from Sigma (St. Louis, MO). Antibodies to SSTR2 (Cat. No.: sc-11609), p21 (sc-756), p63 (sc-8343), histone 4 (H4, sc-10810), HDAC4 (sc-11418), acetylation of histone 4 (AcH4, sc-8660-R), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), with Notch1 (Cat. No.: 4380) from Cell Signaling Technology (Danvers, MA). The plasmids are the kind gifts from Dr. Wu (University of Florida). The cytotoxic CPT-SST was synthesized in our laboratories 38 (link).

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Sun G., Mackey L.V., Coy D.H., Yu C.Y, & Sun L. (2015). The Histone Deacetylase Inhibitor Vaproic Acid Induces Cell Growth Arrest in Hepatocellular Carcinoma Cells via Suppressing Notch Signaling. Journal of Cancer, 6(10), 996-1004.

Publication 2015

Valproic acid sodium salt (VPA) β-actin (sc-1616-HRP) Notch1

Acetylation Actin Antibodies Gifts Histone Plasmids Salt Sstr2 Valproic acid sodium

Corresponding Organization : Fudan University

Other organizations : University of South China

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Variable analysis

independent variables

Valproic acid sodium salt (VPA)

dependent variables

SSTR2
Histone 4 (H4)
HDAC4
Acetylation of histone 4 (AcH4)
Notch1

control variables

β-actin

controls

Positive control: None specified.
Negative control: None specified.

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