Immunoblot Analysis of Cellular Proteins

The methods used for the preparation of cell lysates from in vitro and in vivo samples and for immunoblot analyses have been previously described [20 (link)]. The antibodies used included TFE3 (HPA023881, MilliporeSigma, Burlington, MA, USA), ATP6V1A (17115-1-AP, Proteintech, Rosemont, IL, USA), ATP6V1B1 (A-6427, Invitrogen, Waltham, MA, USA), Histone H3 (4499, Cell Signaling Technology, Danvers, MA, USA), LaminB1 (13435, Cell Signaling Technology), pTfeb (Ser142) (AF3845, Affinity Biosciences, Cincinnati, OH, USA), Atg7 (8558, Cell Signaling Technology), GAPDH (2118S, Cell Signaling Technology), αTubulin (T6199, Sigma-Aldrich, Burlington, MA, USA) and Tfeb (A303-673A, Bethyl Laboratories, Montgomery, TX, USA; sc-48784, Santa Cruz Biotechnology, Dallas, TX, USA).