Vibrio fischeri Bacterial Strains and Plasmids

V. fischeri strains and plasmids used in this study are listed in Table 1, and additional details of their construction are located in Supporting Information. All V. fischeri strains were derived from wild-type strain ES114 (Ruby et al. 2005 (link); Mandel et al. 2008 (link)). Escherichia coli strains used in this work include EC100Dpir+ (Epicentre Biotechnologies, Madison, WI), TAM1 (Active Motif, Carlsbad, CA), β3914 (Le Roux et al. 2007 (link)), π3813 (Le Roux et al. 2007 (link)), CC118 λpir (Herrero et al. 1990 (link)), and GT115 (InvivoGen, San Diego, CA). Oligonucleotides used in this study are listed in Table S1 and were purchased from Integrated DNA Technologies, Inc. (Coralville, IA) (IDT).

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Miyashiro T., Oehlert D., Ray V.A., Visick K.L, & Ruby E.G. (2014). The putative oligosaccharide translocase SypK connects biofilm formation with quorum signaling in Vibrio fischeri. MicrobiologyOpen, 3(6), 836-848.

Publication 2014

GT115

Escherichia coli Oligonucleotides Plasmids Strains

Corresponding Organization :

Other organizations : Pennsylvania State University, University of Wisconsin–Madison, Loyola University Medical Center

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Variable analysis

independent variables

V. fischeri strains and plasmids

dependent variables

Not explicitly mentioned

control variables

Wild-type strain ES114 (Ruby et al. 2005; Mandel et al. 2008)
Escherichia coli strains: EC100Dpir+, TAM1, β3914, π3813, CC118 λpir, GT115

controls

Positive control: Not specified
Negative control: Not specified

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