Apoptosis Expression in Liver Cells

Liver cellular apoptosis expression was studied. TUNEL expression was detected using a ApopTag^® Plus Peroxidase In Situ Apoptosis Detection Kit (CHEMICON International Inc., Temecula, CA, USA) according to the manufacturer’s protocol [2 (link),42 (link)]. Deparaffinized sections were washed with distilled water and treated with Protein Digestion Enzyme for 15 min at 37 °C. The number of positively-stained cells was counted from a total of five hundred hepatocytes in each rat.

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Tsai C.C., Lin Y.J., Yu H.R., Sheen J.M., Lin I.C., Lai Y.J., Tain Y.L., Huang L.T, & Tiao M.M. (2018). Regulation of Leptin Methylation Not via Apoptosis by Melatonin in the Rescue of Chronic Programming Liver Steatosis. International Journal of Molecular Sciences, 19(11), 3565.

Publication 2018

ApopTag® Plus Peroxidase In Situ Apoptosis Detection Kit

Apoptosis Enzyme Hepatocytes Peroxidase Protein digestion Tunel

Corresponding Organization : Chang Gung University

Other organizations : Kaohsiung Medical University

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Variable analysis

independent variables

Protein Digestion Enzyme treatment

dependent variables

TUNEL expression

control variables

Deparaffinized liver sections
Treatment duration (15 min)
Treatment temperature (37 °C)
Counting of positively-stained cells from a total of five hundred hepatocytes in each rat

controls

Positive control: Not specified
Negative control: Not specified

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