If injection of 6-OHDA causes extensive neuronal
damage in the midbrain, two to four weeks after surgery,
animals show successive rotations toward the injection
site in response to apomorphine injection. The number of
these rotations per time unit is a measure of the severity of
neuronal damage in the midbrain. To perform this test, the
rats were first placed in a transparent plastic cylinder (28
cm×38 cm) and after 15 minutes, 0.5 mg/kg body weight
apomorphine hydrochloride was injected into them. After
60 seconds, the number of rotations towards the injection
site or vice versa was recorded at 10-minute intervals for an hour. Finally, the number of rotations toward the
injured side was subtracted from the opposite side, which
indicated the number of net rotations to the opposite side.
Further rotation indicated the severity of the lesion and the
loss of dopaminergic cells (21 (link)).
damage in the midbrain, two to four weeks after surgery,
animals show successive rotations toward the injection
site in response to apomorphine injection. The number of
these rotations per time unit is a measure of the severity of
neuronal damage in the midbrain. To perform this test, the
rats were first placed in a transparent plastic cylinder (28
cm×38 cm) and after 15 minutes, 0.5 mg/kg body weight
apomorphine hydrochloride was injected into them. After
60 seconds, the number of rotations towards the injection
site or vice versa was recorded at 10-minute intervals for an hour. Finally, the number of rotations toward the
injured side was subtracted from the opposite side, which
indicated the number of net rotations to the opposite side.
Further rotation indicated the severity of the lesion and the
loss of dopaminergic cells (21 (link)).
