In order to study expression levels of cytochrome P450 1A (CYP1A) and COX2b, quantitative real-time RT-PCR (RT-qPCR) analysis was carried out [57 (link)]. Total RNA was extracted from larval zebrafish at 55 hpf with TRI-reagent (Sigma-Aldrich, St. Louis, MO). cDNA was prepared from total RNA with ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan). qRT-PCR analysis was performed in Real-Time PCR Detector (LightCycler 96: Roche Life Science, Penzberg, Germany) using Thunderbird qPCR mix containing SYBR Green (Toyobo).
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Quantifying CYP1A and COX2b Expression
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Other organizations : Rakuno Gakuen University, University of Tsukuba
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Variable analysis
independent variables
- Not explicitly mentioned
- Expression levels of cytochrome P450 1A (CYP1A)
- Expression levels of COX2b
- Larval zebrafish at 55 hpf
- TRI-reagent (Sigma-Aldrich, St. Louis, MO) for total RNA extraction
- ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) for cDNA preparation
- Thunderbird qPCR mix containing SYBR Green (Toyobo) for qRT-PCR analysis
- Real-Time PCR Detector (LightCycler 96: Roche Life Science, Penzberg, Germany) for qRT-PCR analysis
- No positive or negative controls were explicitly mentioned in the provided information.
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