Quantification of HIV-1 RNA in plasma and cerebrospinal fluid samples was carried out with clinical routine assays and three different automated platforms during the observation period between January 2011 and October 2022. Until December 2014, the Abbott RealTime HIV-1 assay was used on the Abbott m2000 system. From January 2015 to July 2021, HIV-1 RNA quantification was performed on the Roche CAP/CTM using the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test v.2.0. Since August 2021, quantification has been performed on the Roche c6800 system using the COBAS HIV-1 Test Kit. All analyses were carried out according to the manufacturer’s instructions with a detection limit of 40 copies per ml (Abbott) and 20 copies per ml (Roche).
Ultrasensitive residual viremia was measured in 9 ml of plasma after ultracentrifugation at 170,000g at 4 °C for 30 min, followed by viral RNA extraction using the m2000sp Abbott RealTime HIV-1 assay and laboratory-defined application software from the instrument15 (link). HIV-1 RNA was quantified with a validated in-house calibration curve set with a limit of detection of 0.56 copies per ml.
Ultrasensitive residual viremia was measured in 9 ml of plasma after ultracentrifugation at 170,000g at 4 °C for 30 min, followed by viral RNA extraction using the m2000sp Abbott RealTime HIV-1 assay and laboratory-defined application software from the instrument15 (link). HIV-1 RNA was quantified with a validated in-house calibration curve set with a limit of detection of 0.56 copies per ml.
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