Cell Cycle Analysis of Glioblastoma Cells

The effect of TPL on the cell cycle in glioblastoma was analyzed using the Cell Cycle Staining Kit (Beyotime). And the cell cycle assay was performed as described previously (21 (link)). After treatment with TPL for 24h, U251 cells were trypsinized, fixed in 70% ice-cold ethanol for 24 h and washed with PBS, then incubated with 100 mg/mL RNase and 4 mg/mL propidium iodide (PI) in 500 μl PBS. The cell cycle phase was analyzed using a cytoflex (Beckman Coulter).

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Yuan C., Liao Y., Liao S., Huang M., Li D., Wu W., Quan Y., Li L., Yu X, & Si W. (2023). Triptolide inhibits the progression of Glioblastoma U251 cells via targeting PROX1. Frontiers in Oncology, 13, 1077640.

Publication 2023

Cell Cycle Staining Kit cytoflex

After treatment Assay Cell cycle Cells Cold Ethanol Glioblastoma Propidium iodide Rnase

Corresponding Organization : The First People's Hospital of Zhaoqing

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Variable analysis

independent variables

TPL treatment

dependent variables

Cell cycle phase

control variables

Cell line (U251)
Treatment duration (24h)
Fixation in 70% ice-cold ethanol for 24 h
Staining with RNase (100 mg/mL) and propidium iodide (4 mg/mL) in 500 μl PBS
Cell cycle analysis using a cytoflex (Beckman Coulter)

controls

No explicit mention of positive or negative controls

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