Authenticated MDA-MB-231, MDA-MB-157 and PC3 lines were obtained from ATCC (Teddington, UK). Hs578T cells were kindly provided by Dr Paul Mullan (Centre for Cancer Research and Cell Biology, Queen's University Belfast). The highly invasive clone, MDA-MB-231BO was provided complete with matched parental cells by Prof. Toshiyuki Yoneda (University of Osaka, Japan) [30 (link)] and were cultured in DMEM supplemented with 10% v/v foetal calf serum (FCS) (Invitrogen™ Life Technologies, Paisley, UK). CD44 depleted luciferase-expressing MDA-MB-231 cells from Caliper Life Sciences (Cheshire, UK) were generated in our laboratory by stable transfection with CD44 shRNA or non-targeting shRNA control as previously described [7 (link)] and cultured in DMEM supplemented with 10% v/v FCS, 0.2 μg/ml puromycin. All cell lines were grown to 70% confluence prior to experimentation. Human bone marrow endothelial cells (BMEC) were provided by Dr Babette Weksler (Cornell University, NY) and cultured as previously described [10 (link)].
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