The cell lysing, western blotting and immunoprecipitation were performed as previously described22 (link). The following antibodies were used: OGT, OGA, GAPDH, XBP1, GFAT, CHOP, CEBPB, Snail, pSnail (S246), Vimentin, Twist, N-cadherin and E-cadherin were from Abcam (MA, USA); O-GlcNAcylation antibody (CTD110.6) was from BioLegend (MA, USA). Chemiluminescent detection was performed using ECL kit (GE healthcare, CA, USA).
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