For the in vitro experiments, tibial and femoral bone marrow cells were isolated by dissection. The ends of the bones were cut off to expose the bone marrow and cells were isolated by centrifugation. Cells were re-suspended in growth media (DMEM containing 10% FBS and 1% penicillin-streptomycin) and then plated in 75 ml tissue culture flasks.
To activate Notch signaling in vitro, cells were trypsinized and seeded on pretreated AffiniPure Goat Anti-Human IgG, Fcγ Fragment Specific (Jackson ImmunoResearch 109-005-098) or Jagged-1 (R&D 1277-JG) coated tissue culture plates as described by Kaur et al., and Lee et al.73 ,74 (link)
To activate Notch signaling in vitro, cells were trypsinized and seeded on pretreated AffiniPure Goat Anti-Human IgG, Fcγ Fragment Specific (Jackson ImmunoResearch 109-005-098) or Jagged-1 (R&D 1277-JG) coated tissue culture plates as described by Kaur et al., and Lee et al.73 ,74 (link)
Free full text: Click here
