Total RNA was reverse transcribed using SuperScript II Reverse Transcriptase (LifeTechnologies) according to the manufacturer’s protocol. Quantitative PCR was performed with iTaqTM universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad). Hprt, Rpl13 and Sdha were used as normalization controls (Vandesompele et al., 2002 (link)). Each biological replicate was measured in technical duplicates. The primers used for RT-qPCR are listed in Supplementary Table S3.
Garfa Traoré M., Roccio F., Miceli C., Ferri G., Parisot M., Cagnard N., Lhomme M., Dupont N., Benmerah A., Saunier S, & Delous M. (2023). Fluid shear stress triggers cholesterol biosynthesis and uptake in inner medullary collecting duct cells, independently of nephrocystin-1 and nephrocystin-4. Frontiers in Molecular Biosciences, 10, 1254691.
Other organizations :
Institut des Maladies Génétiques Imagine, Institut Necker Enfants Malades, Fondation pour l’innovation en Cadiométabolisme et Nutrition, Université Sorbonne Paris Nord, Centre Hospitalier Le Vinatier, Centre de Recherche en Neurosciences de Lyon
Total RNA was reverse transcribed using SuperScript II Reverse Transcriptase (LifeTechnologies) according to the manufacturer's protocol
Quantitative PCR was performed with iTaqTM universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad)
Hprt, Rpl13 and Sdha were used as normalization controls (Vandesompele et al., 2002 (link))
Each biological replicate was measured in technical duplicates
controls
Not explicitly mentioned
Not explicitly mentioned
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