Metabolomic analysis was carried out as previously described2 (link). Hearts from the sham and MI-treated groups collected after a 21-day follow-up period were shipped to Metabolon, Inc. (Durham, NC, USA), where they were stored at −80 °C until analysis. Sample preparation, quality control, UPLC‒MS/MS and data analysis were all carried out by Metabolon, Inc. A detailed description is provided in the Supplementary Materials.
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