Immunostaining of BMDMs and Glia

Immunostaining of BMDMs and mixed glial cells was performed as described previously (27 (link)). Immunofluorescent images of cells were visualized at room temperature using a confocal microscope (LSM 700, Zeiss) equipped with a 63×/NA 1.4 objective lens (Plan-Apochromat) and Zen acquisition and analysis software (Zeiss). The images were processed using Photoshop (Adobe) with minimal adjustment of brightness or contrast applied to the entire images.

Free full text: Click here

Chen C.Y., Hung Y.F., Tsai C.Y., Shih Y.C., Chou T.F., Lai M.Z., Wang T.F, & Hsueh Y.P. (2021). Transcriptomic Analysis and C-Terminal Epitope Tagging Reveal Differential Processing and Signaling of Endogenous TLR3 and TLR7. Frontiers in Immunology, 12, 686060.

Publication 2021

LSM 700 Plan-Apochromat Zen acquisition and analysis software

Cells Confocal microscope Glial cells Immunofluorescent Lens

Corresponding Organization : Institute of Molecular Biology, Academia Sinica

Top 5 similar protocols

Variable analysis

independent variables

Immunostaining of BMDMs and mixed glial cells

dependent variables

Immunofluorescent images of cells

control variables

Room temperature
Confocal microscope (LSM 700, Zeiss) equipped with a 63×/NA 1.4 objective lens (Plan-Apochromat) and Zen acquisition and analysis software (Zeiss)

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!