Immunohistochemical Profiling of Formalin-Fixed Tissues

Formalin-fixed tissues were routinely processed into paraffin wax blocks and sections cut for Haemotoxylin and Eosin (H&E) staining and immunohistochemistry. Immunohistochemistry was carried out as described previously (Creedon et al., 2016 (link)). Primary antibodies used were CD31 at 1:800 (Abcam, ab28364), ERG (Dako, IR659, ready to use, 200 μl per slide), p53 at 1:2000 (Leica BioSystems, NCL-L-p53-CM5p), PDGFR-β at 1:100 (CST, 3169S), and VE-cadherin at 1:4000 (Abcam, ab33168).

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Salter D.M., Griffin M., Muir M., Teo K., Culley J., Smith J.R., Gomez-Cuadrado L., Matchett K., Sims A.H., Hayward L., Henderson N.C, & Brunton V.G. (2019). Development of mouse models of angiosarcoma driven by p53. Disease Models & Mechanisms, 12(7), dmm038612.

Publication 2019

ab28364 ab33168

Antibodies Cadherin 1 Eosin Formalin Haemotoxylin Immunohistochemistry Paraffin wax Pdgfr β Tissues

Corresponding Organization :

Other organizations : Edinburgh Cancer Research, Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Immunohistochemistry staining patterns for CD31, ERG, p53, PDGFR-β, and VE-cadherin

control variables

Formalin-fixed tissues
Paraffin wax blocks
Haemotoxylin and Eosin (H&E) staining

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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