Antibody Characterization for Cytoskeletal Analysis

The GT335 (1:500 dilution) and polyE (1:200 dilution) antibodies were obtained from AdipoGen (San Diego, CA); anti-anti-acetylated α-tubulin (1:1000 dilution) and anti-polyglutamylated tubulin B3 (1:500 dilution) were obtained from Sigma-Aldrich; anti-detyrosinated antibody was obtained from Abcam; anti-Xpress antibody (1:200 dilution) was procured from Invitrogen. Anti-RPGR antibody (1:500 dilution) was raised against an N-terminal epitope, which is common to both RPGR^const and RPGR^ORF15 isoforms. Detailed characterization of this antibody was previously reported (Ghosh et al., 2010 (link); Rao et al., 2015 (link)).

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Rao K.N., Anand M, & Khanna H. (2016). The carboxyl terminal mutational hotspot of the ciliary disease protein RPGRORF15 (retinitis pigmentosa GTPase regulator) is glutamylated in vivo. Biology Open, 5(4), 424-428.

Publication 2016

GT335 polyE anti-Xpress antibody

Anti antibody Antibodies Antibody Dilution Epitope Isoforms Tubulin α tubulin

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Variable analysis

independent variables

GT335 (1:500 dilution)
PolyE (1:200 dilution)
Anti-acetylated α-tubulin (1:1000 dilution)
Anti-polyglutamylated tubulin B3 (1:500 dilution)
Anti-detyrosinated antibody
Anti-Xpress antibody (1:200 dilution)
Anti-RPGR antibody (1:500 dilution)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None specified
Negative control: None specified

Annotations

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