Up to 2 μl of a 1 : 1000 nanoparticle solution was placed on top of each LN slice. A coverslip was placed on the slice and stayed on by means of capillary forces. Movies were captured using a 63× objective on a ZEISS Axioscope 5 microscope and by using Zeiss software (ZEN lite) at a temporal resolution of 30 or 50 ms for 20 s. Mean squared displacement (MSD) and nanoparticle trajectory was calculated using MATLAB, with a minimum of 10 frames for each particle.21 (link) MSD was calculated using Δr2(τ) = [x(t + τ) − x(t)]2 + [y(t + τ) − y(t)]2 with at least 70 particles.
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Nanoparticle Diffusion in Tissue Slices
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Capillary
Frames
Microscope
Corresponding Organization : University of Maryland, College Park
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Variable analysis
independent variables
- Volume of nanoparticle solution placed on LN slice (up to 2 μl)
- Mean squared displacement (MSD) of nanoparticles
- Nanoparticle trajectory
- Nanoparticle solution concentration (1:1000)
- Temporal resolution of movies (30 or 50 ms)
- Duration of movies (20 s)
- Minimum number of frames per particle (10)
- Microscope objective (63×)
- Microscope (ZEISS Axioscope 5)
- Imaging software (Zeiss ZEN lite)
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