The MCF-7 cell lines were first grown in DMEM supplemented with 10% FBS to test migration assay, then seeded in 6-well plates.19 (link) Next, the width of each well is scratched by sterile pipette tip (1 mm). The wells are washed by the culture medium and the picture of wounds is taken at 0 hour (NIKON, Inverted research microscope). Thereafter, medium was replaced with different concentrations of PLGA-PTX-VitD3 co-delivery NPs, PTX and PLGA-PTX (IC50 concentration) and the cells are allowed to migrate for 24 hours. After 24 hours, the picture of wound is taken from the same regions. The rate of migration (RM) is analyzed by measuring the distance between the scratch edges with digitizer software. The RM is calculated by following equation: RM=(Wi-Wf)/t.20 (link)
Free full text: Click here