HTR-8/SVneo cells were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were grown at 37 °C, 5% CO2.
The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, and negative controls (si-NC and oe-NC) were purchased from HonorGene (Changsha, China). The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, si-SREBP1, and negative controls were transfected into cells by Lipofectamine 2000. Rosiglitazone (122320-73-4, MedChemExpress, USA) is an agonist of PPARγ. Erastin (HY-15763, MedChemExpress, USA) is an agonist of ferroptosis and ferrostatin-1 (ferr1, HY-100579, MedChemExpress, USA) is an inhibitor. After treatment with 1 μM Rosiglitazone, 1 μM ferr1, and 1 μmol erastin for 24 h, cells were subjected to hypoxia for 24 h. As described earlier25 (link), the cells were placed under 2% O2 for 24 h to establish a hypoxic cell model. Cells in the NC group were given normoxic conditions with 20% O2. Cells were observed by a transmission electron microscope (jem-1400).
The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, and negative controls (si-NC and oe-NC) were purchased from HonorGene (Changsha, China). The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, si-SREBP1, and negative controls were transfected into cells by Lipofectamine 2000. Rosiglitazone (122320-73-4, MedChemExpress, USA) is an agonist of PPARγ. Erastin (HY-15763, MedChemExpress, USA) is an agonist of ferroptosis and ferrostatin-1 (ferr1, HY-100579, MedChemExpress, USA) is an inhibitor. After treatment with 1 μM Rosiglitazone, 1 μM ferr1, and 1 μmol erastin for 24 h, cells were subjected to hypoxia for 24 h. As described earlier25 (link), the cells were placed under 2% O2 for 24 h to establish a hypoxic cell model. Cells in the NC group were given normoxic conditions with 20% O2. Cells were observed by a transmission electron microscope (jem-1400).
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