The tracking of the rotation of the optically anisotropic particles
is based on a method reported by Niggel et al.37 (link) First, the center of the particle is found in each frame
of a time series of standard wide-field epi-fluorescence microscopy
images (Axio Observer D1, 40× NA = 0.6 objective, Filter Set
09, Zeiss, Germany, 89 North Photofluor II, USA, Zyla 4.2, Andor,
UK, acquisition rate: 25 frames per second, 2048 × 2048 pixels).
Then, using image correlation, a reference image is compared with
sequentially rotated images of a subsequent frame to find the most
probable change in angular orientation. The instantaneous rotation
of the particle can be extracted and synchronized with the friction
force, as seen inFigure 1 F–H. Alternatively, the cumulative rotation in one
scan can be determined and compared to the theoretical rolling without
slip of a particle of the same diameter over the same distance. The
rotation can be displayed as a percentage compared to pure rolling
without slip, as shown later in the article.
is based on a method reported by Niggel et al.37 (link) First, the center of the particle is found in each frame
of a time series of standard wide-field epi-fluorescence microscopy
images (Axio Observer D1, 40× NA = 0.6 objective, Filter Set
09, Zeiss, Germany, 89 North Photofluor II, USA, Zyla 4.2, Andor,
UK, acquisition rate: 25 frames per second, 2048 × 2048 pixels).
Then, using image correlation, a reference image is compared with
sequentially rotated images of a subsequent frame to find the most
probable change in angular orientation. The instantaneous rotation
of the particle can be extracted and synchronized with the friction
force, as seen in
scan can be determined and compared to the theoretical rolling without
slip of a particle of the same diameter over the same distance. The
rotation can be displayed as a percentage compared to pure rolling
without slip, as shown later in the article.
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