Western Blot Analysis of HCMV Proteins

SDS-PAGE and standard Western blot analysis, based on equalized amounts of the total cell lysates, were performed as described previously in [64 (link)]. The antibodies used for staining were mAb-HA (H9658, Sigma-Aldrich, USA), pAb-Flag (F7425, Sigma-Aldrich), mAb-IE1p72 (P63-27) [65 (link)], and mAb-pp28 (both kindly provided by William J. Britt, Dept. Pediatrics Microbiol., Univ. Alabama, Birmingham, AL, USA), mAb-UL44 (kindly provided by Bodo Plachter, Virology, Univ. Mainz, Germany), and mAb-actin (A5441, Sigma-Aldrich).

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Yu D., Wagner S., Schütz M., Jeon Y., Seo M., Kim J., Brückner N., Kicuntod J., Tillmanns J., Wangen C., Hahn F., Kaufer B.B., Neipel F., Eickhoff J., Klebl B., Nam K, & Marschall M. (2024). An Antiherpesviral Host-Directed Strategy Based on CDK7 Covalently Binding Drugs: Target-Selective, Picomolar-Dose, Cross-Virus Reactivity. Pharmaceutics, 16(2), 158.

Publication 2024

mAb-HA pAb-Flag mAb-UL44

Corresponding Organization : Friedrich-Alexander-Universität Erlangen-Nürnberg

Other organizations : Qurient (South Korea), Freie Universität Berlin, Lead Discovery Center (Germany), UiT The Arctic University of Norway

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Variable analysis

independent variables

Antibodies used for staining: mAb-HA (H9658, Sigma-Aldrich, USA), pAb-Flag (F7425, Sigma-Aldrich), mAb-IE1p72 (P63-27), mAb-pp28, mAb-UL44, mAb-actin (A5441, Sigma-Aldrich)

dependent variables

Protein expression levels (as measured by SDS-PAGE and standard Western blot analysis)

control variables

Total cell lysates (equalized amounts used for analysis)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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