Streptozotocin-Induced Diabetes Model with Adjuvants

Streptozotocin (SIGMA) was diluted in citrate sodium buffer, and administered intraperitoneally at the dose of 150 mg/kg of body weight (BW) twice with an interval of 4 days^{26 (link)}. Mice were used for experiments 4 weeks after the treatments. Leupeptin (Wako) was diluted in phosphate-buffered saline, and administered intraperitoneally at the dose of 40 mg/kg of BW 4 h before sacrifice^{56 (link)}. Calcein (Dojindo) was diluted in NaHCO3 solution, and administered subcutaneously at the dose of 8 mg/kg of BW twice, 8 days and 2 days before sacrifice^{57 (link)}. AS1842856, or 5-amino-7-(cyclohexylamino)−1-ethyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (Universal Biologicals), was diluted in 6% β-cyclodextrin (Sigma), and administered orally at the dose of 100 mg/kg of BW^{32 (link)} once daily for 4 weeks.