The chloride salt of aquacobalamin
([H2OCbl]Cl), dicyanocobinamide [(CN)2Cbi],
and potassium formate (HCOOK) were purchased from Sigma and used as
obtained. Diaquacobinamide {[(H2O)2Cbi]2+} was prepared by adding NaBH4 to an aqueous solution
of (CN)2Cbi, loading the reaction mixture on a C18 SepPack
column, washing it with doubly distilled H2O, and eluting
the product with methanol, as described in previous reports.24 (link),30 (link) Co(II)Cbl and Co(II)Cbi+ were prepared by adding a small
volume of saturated HCOOK to degassed solutions of H2OCbl+ and (H2O)2Cbi2+, respectively,
and the progress of the reduction was monitored spectrophotometrically.
([H2OCbl]Cl), dicyanocobinamide [(CN)2Cbi],
and potassium formate (HCOOK) were purchased from Sigma and used as
obtained. Diaquacobinamide {[(H2O)2Cbi]2+} was prepared by adding NaBH4 to an aqueous solution
of (CN)2Cbi, loading the reaction mixture on a C18 SepPack
column, washing it with doubly distilled H2O, and eluting
the product with methanol, as described in previous reports.24 (link),30 (link) Co(II)Cbl and Co(II)Cbi+ were prepared by adding a small
volume of saturated HCOOK to degassed solutions of H2OCbl+ and (H2O)2Cbi2+, respectively,
and the progress of the reduction was monitored spectrophotometrically.
