Flow Cytometry of Blood and Tumor Samples

Blood and tumor samples were processed for flow cytometry as described previously.25 31 32 (link) Cells were stained with the following fluorochrome-conjugated monoclonal antibodies: CD3-FITC (clone HIT3a, BioLegend), CD56-PE (clone HCD56, BioLegend), and CD45-BV510 (clone HI30, BioLegend). Live/dead staining was performed using Fixable Viability Dye 780 (eBioscience #65-0865-14). Flow cytometry results were acquired using a BD LSRFortessa flow cytometer (Becton Dickinson, San Jose, California, USA) and analyzed using FlowJo Software (Becton Dickinson, San Jose, California, USA).

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Perry L.M., Cruz S.M., Kleber K.T., Judge S.J., Darrow M.A., Jones L.B., Basmaci U.N., Joshi N., Settles M.L., Durbin-Johnson B.P., Gingrich A.A., Monjazeb A.M., Carr-Ascher J., Thorpe S.W., Murphy W.J., Eisen J.A, & Canter R.J. (2023). Human soft tissue sarcomas harbor an intratumoral viral microbiome which is linked with natural killer cell infiltrate and prognosis. Journal for Immunotherapy of Cancer, 11(1), e004285.

Publication 2023

CD3-FITC (clone HIT3a, CD56-PE (clone HCD56, CD45-BV510 (clone HI30, Fixable Viability Dye 780 BD LSRFortessa flow cytometer

Blood Cells Clone Fitc Flow cytometry Fluorochrome Monoclonal antibodies Tumor

Corresponding Organization : University of California, Davis

Other organizations : Baylor Scott & White Health

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Variable analysis

independent variables

Blood and tumor samples

dependent variables

Flow cytometry results

control variables

Cells were stained with the following fluorochrome-conjugated monoclonal antibodies: CD3-FITC, CD56-PE, and CD45-BV510
Live/dead staining was performed using Fixable Viability Dye 780

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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