Standard/Material preparation and use was done, as described previously [19 (link),20 (link)]. Briefly, the cannabinoid standards at a concentration of 1 mg/mL in methanol used in this study included THC (34067; Restek, Bellefonte, PA, USA), CBC (34092; Restek), CBG (34091; Restek) and CBN (34010; Restek). Inverse agonists (IA) to CB1 and CB2 were AM251 (ab120088; Abcam, Cambridge, UK) and SR144528 (ab146185; Abcam), respectively. The TRPA1 blocker used was HC-030031 (ab120554; Abcam). TRPV1 and TRPV2 antagonists were ab141772 (Abcam) and Tranilast 1098/10 (Abcam), respectively. All IAs, the blocker and antagonists were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mM and used for cell treatment at a final concentration of 10 µM. Chemotherapy solutions for synergy tests included niraparib (AG0038ZU; Angene, Nanjing, China) and gemcitabine (461060010; Acros Organics, Beijing, China) both dissolved in DMSO. Solutions for induction of malignant features [21 (link)] included IL-1β (200-01B; PeproTech, Cranbury, NJ, USA) and TNFα (300-01A; PeproTech) both dissolved in pure water. Solvents (methanol and/or DMSO) were used as a negative control and niraparib was used as a positive control in all the biological assays. The control in any given experiment was set at a concentration to match the vehicle concentration in the highest concentration treatment.
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