The highly stable pristine C60 aqueous colloid solution (2.6 mg/mL, purity > 99.5%,) was prepared as described in [31 (link),32 (link)]. Ber was dissolved in a distilled water at initial concentration of 1 mg/mL. C60 and Ber solutions were mixed in a 2:1 C60-Ber molar ratio (208:104 µM), and the formation of a stable noncovalent nanocomplex was confirmed by the UV–Vis spectroscopy, atomic force microscopy (AFM, Solver Pro M system, NT-MDT, Moscow, Russia), and dynamic light scattering (DLS) measurements as described in [24 (link)].
Horak I., Prylutska S., Krysiuk I., Luhovskyi S., Hrabovsky O., Tverdokhleb N., Franskevych D., Rumiantsev D., Senenko A., Evstigneev M., Drobot L., Matyshevska O., Ritter U., Piosik J, & Prylutskyy Y. (2021). Nanocomplex of Berberine with C60 Fullerene Is a Potent Suppressor of Lewis Lung Carcinoma Cells Invasion In Vitro and Metastatic Activity In Vivo. Materials, 14(20), 6114.
Atomic force microscopy Colloid Light scattering measurementsMolar Spectroscopy
Corresponding Organization : Gdańsk Medical University
Other organizations :
Palladin Institute of Biochemistry, D.F. Chebotarev Institute of Gerontology, Leibniz Institute of Polymer Research, Taras Shevchenko National University of Kyiv, Belgorod National Research University, Technische Universität Ilmenau
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