Fatty Acid Methyl Esters Quantification

Harvested cell pellets and cell-free culture media were placed in tube with Teflon caps after freeze-drying. Methylating mixture [MeOH:Benzene:DMP (2,2-Dimethoxy-propane):H2SO4 = 39:20:5:2] and heptane were added to the tube and incubated at 80℃ for 2 h. After cooling the tubes at room temperature, two phases were observed. The upper phase containing fatty acid methyl esters (FAMEs) was extracted to conduct gas chromatography (Agilent Technologies, inc. Santa Clara, CA, USA) at NICEM of Seoul National University [32 (link)]. Quantitative validation of FAMEs was also performed at NICEM.

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Hwang S.H., Yang Y., Jung J.H, & Kim Y. (2022). Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition. Cancer Cell International, 22, 404.

Publication 2022

gas chromatography

Benzene Cell Cell culture Culture media Esters Fatty acid Gas chromatography Heptane Pellets Propane Teflon

Corresponding Organization :

Other organizations : Seoul National University

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Variable analysis

independent variables

Methylating mixture [MeOH:Benzene:DMP (2,2-Dimethoxy-propane):H2SO4 = 39:20:5:2]
Heptane

dependent variables

Fatty acid methyl esters (FAMEs)

control variables

Harvested cell pellets and cell-free culture media were placed in tube with Teflon caps after freeze-drying
Incubation at 80°C for 2 h
Gas chromatography (Agilent Technologies, inc. Santa Clara, CA, USA) at NICEM of Seoul National University

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