Serum Lipid Profiling Protocol

All the samples from each participant were stored in the Shiraz Endocrinology Research Center after overnight fasting. The samples were collected at 8 a.m. Serum total cholesterol, HDL-C, and TG values were assessed by enzymatic reagents (Biosystems, Barcelona, Spain) with an A-25 Biosystem Autoanalyser. The Friedwald equation was used to indirectly measure LDL concentrations from calculated TG, HDLc, and TC [20 (link)]. Non-HDL-C was calculated by detracting HDL-C from total cholesterol [21 (link)]. The coefficient of variation (CV%) for TG, TC, and HDL methods was 1.7–2.6%, 1–1.9.5%, and 1.3–1.5%, respectively.