SK-MEL-2 cells (gift from David Drubin, UC Berkeley, described in Doyon et al. [2011] (link)) were cultured adherently in DMEM (Gibco, no. 10565-018), supplemented with 10% (v/v) FBS (Gibco, no. 10270-106), and ZellShield (Biochrom AG) at 37°C, under an atmosphere with 5% CO2 and 100% humidity.
U2OS cells (#300174; Cell Line Services) were cultured adherently as described previously (Thevathasan et al., 2019 (link)) in DMEM (Gibco, no. 11880-028) supplemented with 10% FBS (Gibco, no. 10270-106), 1x GlutaMAX (Gibco, no. 35050-038), nonessential amino acids (Gibco, no. 11140-035), and ZellShield (Biochrom AG) at 37°C under an atmosphere with 5% CO2 and 100% humidity.
3T3 mouse fibroblasts (gift from Alba Diz-Muñoz, EMBL Heidelberg) were cultured adherently in DMEM (4.5 g/l D-Glucose) supplemented with 1× MEM NEAA (Gibco, no. 11140-035), 1× GlutaMAX (Gibco, no. 35050-038), and 10% (v/v) FBS (Gibco, no. 10270-106) at 37°C under an atmosphere with 5% CO2, 100% humidity.
U2OS cells (#300174; Cell Line Services) were cultured adherently as described previously (Thevathasan et al., 2019 (link)) in DMEM (Gibco, no. 11880-028) supplemented with 10% FBS (Gibco, no. 10270-106), 1x GlutaMAX (Gibco, no. 35050-038), nonessential amino acids (Gibco, no. 11140-035), and ZellShield (Biochrom AG) at 37°C under an atmosphere with 5% CO2 and 100% humidity.
3T3 mouse fibroblasts (gift from Alba Diz-Muñoz, EMBL Heidelberg) were cultured adherently in DMEM (4.5 g/l D-Glucose) supplemented with 1× MEM NEAA (Gibco, no. 11140-035), 1× GlutaMAX (Gibco, no. 35050-038), and 10% (v/v) FBS (Gibco, no. 10270-106) at 37°C under an atmosphere with 5% CO2, 100% humidity.
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