The hiPSC cell line SB Ad3.1 was obtained from the StemBANCC consortium via the Human Biomaterials Resource Centre, University of Birmingham [39 (link)]. Cells were grown in mTeSR1 basal medium supplemented with mTeSR1 5× supplement (Stemcell Technologies, UK), 100 U/ml penicillin and 100 μg/ml streptomycin on cell culture plates coated with DMEM/F12 (Sigma-Aldrich, UK) supplemented with hESC-qualified Matrigel, diluted according to the manufacturer’s instructions and lot number (Corning, UK). All cell lines were maintained at 37°C and 5% CO2 and routinely tested negative for mycoplasma.
Mattis K.K., Krentz N.A., Metzendorf C., Abaitua F., Spigelman A.F., Sun H., Ikle J.M., Thaman S., Rottner A.K., Bautista A., Mazzaferro E., Perez-Alcantara M., Manning Fox J.E., Torres J.M., Wesolowska-Andersen A., Yu G.Z., Mahajan A., Larsson A., MacDonald P.E., Davies B., den Hoed M, & Gloyn A.L. (2023). Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression. Diabetologia, 66(4), 674-694.
Other organizations :
Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Wellcome Centre for Human Genetics, Science for Life Laboratory, Uppsala University, University of Alberta, Stanford University
Culture medium (mTeSR1 basal medium supplemented with mTeSR1 5× supplement)
Cell culture plates (coated with DMEM/F12 supplemented with hESC-qualified Matrigel)
Incubation conditions (37°C, 5% CO2)
Mycoplasma testing (routinely tested negative)
controls
Positive control: Not specified
Negative control: Not specified
Annotations
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