Lipidomic Analysis: Standards and Solvents

LC-MS grade acetonitrile, methanol, water, isopropyl alcohol, and HPLC grade methanol, and chloroform were purchased from Thermo Fisher Scientific (Fairlawn, NJ, USA). LC/MS grade eluent buffers, ammonium acetate, and acetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Diacylglycerol (DG, 10:0/10:0), triacylglycerol (TG, 17:0/17:0/17:0), phosphatidylcholine (PC, 10:0/10:0), phosphatidylethanolamine (PE, 10:0/10:0), sphingomyelin (SM, 18:1d/17:0), and ceramide (CER, C17) for internal standards were acquired from Avanti Polar Lipids (Alabaster, AL, USA). Diacylglycerol (DG, 16:0/16:0), phosphatidylcholine (PC, 16:0/16:0), phosphatidylethanolamine (PE, 16:0/16:0), lyso-phosphatidylcholine (lyso-PC, 18:0), lyso-phosphatidylethanolamine (lyso-PE 16:0), triacylglycerol (TG, 17:0/17:0/17:0), sphingomyelin (SM, 18:1d/17:0), and ceramide (CER, C17) as standards for retention time were obtained from Avanti Polar Lipids (Alabaster, AL, USA).

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Hong S.H., Lee J.Y., Seo S., Shin B., Jeong C.H., Bae E., Kim J., Lee D., An B., Shim M., Shin J.H., Lee D.K., Kim Y.J, & Han S.B. (2023). Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth. Metabolites, 13(2), 177.

Publication 2023

LC-MS grade acetonitrile methanol isopropyl alcohol HPLC grade methanol chloroform ammonium acetate acetic acid Diacylglycerol triacylglycerol phosphatidylcholine phosphatidylethanolamine sphingomyelin lyso-phosphatidylcholine lyso-phosphatidylethanolamine

Acetic acid Acetonitrile Alabaster Ammonium acetate Buffers Ceramide Chloroform Diacylglycerol Hplc Isopropyl alcohol Lipids Lyso pc Methanol Phosphatidylcholine Phosphatidylethanolamine Retention Sphingomyelin Triacylglycerol

Corresponding Organization : Ewha Womans University Medical Center

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Variable analysis

independent variables

LC-MS grade acetonitrile, methanol, water, isopropyl alcohol, and HPLC grade methanol, and chloroform

dependent variables

Not explicitly mentioned

control variables

LC/MS grade eluent buffers, ammonium acetate, and acetic acid
Diacylglycerol (DG, 10:0/10:0), triacylglycerol (TG, 17:0/17:0/17:0), phosphatidylcholine (PC, 10:0/10:0), phosphatidylethanolamine (PE, 10:0/10:0), sphingomyelin (SM, 18:1d/17:0), and ceramide (CER, C17) for internal standards
Diacylglycerol (DG, 16:0/16:0), phosphatidylcholine (PC, 16:0/16:0), phosphatidylethanolamine (PE, 16:0/16:0), lyso-phosphatidylcholine (lyso-PC, 18:0), lyso-phosphatidylethanolamine (lyso-PE 16:0), triacylglycerol (TG, 17:0/17:0/17:0), sphingomyelin (SM, 18:1d/17:0), and ceramide (CER, C17) as standards for retention time

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