Conditional Knockout Mouse Models for AML Research

Cbl^f/f;Cbl-b^f/f mice were generated as described (Goetz et al. 2016 (link)) and crossed to cre^ERT2 mice from Jackson Laboratories. Lnk^−/− mice were generously provided by Dr. Tony Pawson and Dr. Laura Velazquez (Samuel Lunenfeld Research Institute, Toronto, Canada). BM- or peripheral blood-derived MNCs from deidentified AML patients were obtained from the Stem Cell and Xenograft Core Facility at University of Pennsylvania. TF-1 and HEL cells were purchased from American Type Culture Collection. HEL cells were cultured in RPMI with 10% bovine calf serum. TF-1 cells were maintained in RPMI supplemented with 10% CS and 2 ng/mL hGM-CSF (PeproTech).

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Lv K., Jiang J., Donaghy R., Riling C.R., Cheng Y., Chandra V., Rozenova K., An W., Mohapatra B.C., Goetz B.T., Pillai V., Han X., Todd E.A., Jeschke G.R., Langdon W.Y., Kumar S., Hexner E.O., Band H, & Tong W. (2017). CBL family E3 ubiquitin ligases control JAK2 ubiquitination and stability in hematopoietic stem cells and myeloid malignancies. Genes & Development, 31(10), 1007-1023.

Publication 2017

creERT2 mice TF-1 cells hGM-CSF

Blood Bovine Cblf Cells Mice Patients Serum Stem cell Xenograft

Corresponding Organization :

Other organizations : Children's Hospital of Philadelphia, University of Pennsylvania, Progenra (United States), University of Nebraska Medical Center, University of Western Australia

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Variable analysis

independent variables

Generating Cbl^f/f;Cbl-b^f/f mice and crossing them to cre^ERT2 mice
Obtaining BM- or peripheral blood-derived MNCs from deidentified AML patients

dependent variables

Unspecified

control variables

Culturing HEL cells in RPMI with 10% bovine calf serum
Maintaining TF-1 cells in RPMI supplemented with 10% CS and 2 ng/mL hGM-CSF (PeproTech)

positive controls

None specified

negative controls

None specified

Annotations

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