All the mice used here have the C57BL/6 background. Atg7fl/fl mice were described previously (20 (link)). LysMcre/cre mice were purchased from Jackson Laboratories. All the experiments were performed as approved by IACUC. Antibodies against CD45, CD11b, F4/80, TLR4, TLR2, CD4, CD3, CD11c, Siglec F, CD200R, Ly6G and Ly6C were purchased from BioLegend. Dectin-1 and dectin-2 antibodies were from AbD Serotec. Antibodies against TREM2 and MARCO were purchased by R&D. MitoSOX and Dihydroethidium (DHE) were purchased from Life Technologies. An ROS inhibitor, N-acetyl-L-cyteine (NAC) was purchased from Sigma.
Protocol detail
Macrophage ROS Metabolism Regulation
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Corresponding Organization : Duke Medical Center
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Variable analysis
independent variables
- Genotype (Atg7 and LysM)
- Expression of CD45, CD11b, F4/80, TLR4, TLR2, CD4, CD3, CD11c, Siglec F, CD200R, Ly6G and Ly6C
- Expression of Dectin-1 and Dectin-2
- Expression of TREM2 and MARCO
- Reactive oxygen species (ROS) levels (measured by MitoSOX and Dihydroethidium)
- C57BL/6 mouse background
- Positive control: None specified
- Negative control: None specified
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