Comprehensive Analysis of Tumor-Infiltrating Myeloid Cells

CEA_526–533 and HIV-GAG tetramer staining were performed as previously described (1 (link)). To analyze TIMs, 21-day-old MC38-CEA tumors were harvested and enzymatically digested to obtain a single-cell suspension (1 (link)). Anti-CD11b Alexa Fluor 700 clone M1/70 and anti-Gr1 APC-Cy7 clone RB6-8C5 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CXCL9 Alexa Fluor 647 clone MIG-2F5.5, anti-CD105 PerCp-Cy5.5 clone MJ7/18, and anti-CD31 Pacific Blue clone 390 were purchased from BioLegend (San Diego, CA). Anti-CD45 eFluor 605NC clone 30-F11 and anti-FAS-L PerCP-eFluor 710 clone MFL3 were purchased from eBioscience (San Diego, CA). At least 3 × 10⁵ live cells were acquired with an LSR-II flow cytometer (BD Biosciences) and data were analyzed with FlowJo software for PC (TreeStar Inc., Ashland, OR).

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Farsaci B., Donahue R.N., Coplin M.A., Grenga I., Lepone L.M., Molinolo A.A, & Hodge J.W. (2014). Immune Consequences of Decreasing Tumor Vasculature with Antiangiogenic Tyrosine Kinase Inhibitors in Combination with Therapeutic Vaccines. Cancer immunology research, 2(11), 1090-1102.

Publication 2014

Anti-CD11b Alexa Fluor 700 clone M1/70 LSR-II flow cytometer

Alexa fluor 647 Cd11b Cells Clone Cy5 5 Fas l Tetramer Tims Tumors

Corresponding Organization : Center for Cancer Research

Other organizations : National Institute of Dental and Craniofacial Research

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Variable analysis

independent variables

CEA_526–533 and HIV-GAG tetramer staining
Anti-CD11b Alexa Fluor 700 clone M1/70
Anti-Gr1 APC-Cy7 clone RB6-8C5
Anti-CXCL9 Alexa Fluor 647 clone MIG-2F5.5
Anti-CD105 PerCp-Cy5.5 clone MJ7/18
Anti-CD31 Pacific Blue clone 390
Anti-CD45 eFluor 605NC clone 30-F11
Anti-FAS-L PerCP-eFluor 710 clone MFL3

dependent variables

TIMs (Tumor-infiltrating myeloid cells)

control variables

21-day-old MC38-CEA tumors

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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