Necropsy and Immunohistochemistry for Nipah Virus

Necropsy was performed on all subjects. Tissue samples of all major organs were collected for histopathologic and immunohistochemical examination, immersion-fixed in 10% neutral buffered formalin, and processed for histopathology as previously described (20 (link)). For immuno-histochemistry, specific anti-NiV immunoreactivity was detected using an anti-NiV N protein rabbit primary antibody at a 1:5000 dilution (12 ). In brief, tissue sections were processed for immunohistochemistry using the Dako Autostainer. Secondary antibody used was biotinylated goat anti-rabbit IgG (Vector Laboratories) at 1:200 followed by Dako LSAB2 streptavidin–horseradish peroxidase. Slides were developed with Dako DAB chromagen and counterstained with hematoxylin. Nonimmune rabbit IgG was used as a negative control.

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Geisbert T.W., Mire C.E., Geisbert J.B., Chan Y.P., Agans K.N., Feldmann F., Fenton K.A., Zhu Z., Dimitrov D.S., Scott D.P., Bossart K.N., Feldmann H, & Broder C.C. (2014). Therapeutic Treatment of Nipah Virus Infection in Nonhuman Primates with a Neutralizing Human Monoclonal Antibody. Science translational medicine, 6(242), 242ra82.

Publication 2014

Autostainer biotinylated goat anti-rabbit IgG LSAB2 streptavidin–horseradish peroxidase DAB chromagen

Anti antibody Anti igg Antibody Dilution Formalin Goat Hematoxylin Horseradish peroxidase Immersion Immunohistochemistry N protein Necropsy Rabbit Streptavidin Tissue Vector

Corresponding Organization : Uniformed Services University of the Health Sciences

Other organizations : National Institutes of Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases

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control variables

Tissue samples of all major organs were collected for histopathologic and immunohistochemical examination
Tissue samples were immersion-fixed in 10% neutral buffered formalin and processed for histopathology
Specific anti-NiV immunoreactivity was detected using an anti-NiV N protein rabbit primary antibody at a 1:5000 dilution
Nonimmune rabbit IgG was used as a negative control

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