Western blotting was performed as previously described.21 (link) The following primary antibodies were used: APOC1 (1:1000, ab198288), cyclin B1 (1:5000, ab32053), cyclin D1 (1:5000, ab134175), caspase-9 (1:1000, ab202068), BCL-2 (1:500, ab32124), ERK1/2 (1:500, ab196883), phospho-ERK1/2 (1:500, ab65142), and GADPH (1:2500, ab9485) were purchased from Abcam Inc. Phospho-p38 (1:1000, #9211), p38 (1:1000, #9212), phospho-JNK (1:1000, #9251) and JNK (1:1000, #9252) were purchased from Cell Signaling Technology (Boston, MA, USA). Goat anti-rabbit IgG H&L (1:2000, ab6721, Abcam) was used as a second antibody.
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- Primary antibodies used for western blotting: APOC1, cyclin B1, cyclin D1, caspase-9, BCL-2, ERK1/2, phospho-ERK1/2, GADPH, phospho-p38, p38, phospho-JNK, JNK
- Not explicitly mentioned
- Goat anti-rabbit IgG H&L used as a secondary antibody
- No positive or negative controls were explicitly mentioned in the input protocol.
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