Measurement of Inflammatory and Autoantibody Markers

Serum IL-6 concentrations were measured by Mouse IL-6 High Sensitivity ELISA according to the manufacturer’s protocol (eBioscience). Serum anti-GPI IgM and IgG antibodies were measured by ELISA according to a previously reported protocol with slight modifications39 (link). A serum mixture of 4 WT mice 14 days after immunization was used as 1000 unit (U) standard of the anti-GPI antibody. Serum anti-CCP2 antibodies were measured by Mesacup CCP2 test (MBL) in accordance to the previous study40 (link).

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Seri Y., Shoda H., Suzuki A., Matsumoto I., Sumida T., Fujio K, & Yamamoto K. (2015). Peptidylarginine deiminase type 4 deficiency reduced arthritis severity in a glucose-6-phosphate isomerase-induced arthritis model. Scientific Reports, 5, 13041.

Publication 2015

Mouse IL-6 High Sensitivity ELISA

Anti antibodies Anti antibody Anti igm Elisa Igg antibodies Immunization Mice Mouse il 6 Sensitivity Serum

Corresponding Organization :

Other organizations : The University of Tokyo, RIKEN, University of Tsukuba

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Variable analysis

independent variables

Serum IL-6 concentrations measured by Mouse IL-6 High Sensitivity ELISA
Serum anti-GPI IgM and IgG antibodies measured by ELISA
Serum anti-CCP2 antibodies measured by Mesacup CCP2 test

dependent variables

Serum IL-6 concentrations
Serum anti-GPI IgM and IgG antibody levels
Serum anti-CCP2 antibody levels

control variables

Serum mixture of 4 WT mice 14 days after immunization used as 1000 unit (U) standard of the anti-GPI antibody

controls

Positive control: Serum mixture of 4 WT mice 14 days after immunization used as 1000 unit (U) standard of the anti-GPI antibody

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