LDH activity was measured using a specific LDH Activity Assay Kit (Sigma Aldrich, St. Louis, MI, USA) at 3, 7, 14, 21, and 28 days of cell culture. All conditions were tested in duplicate. The culture medium was reserved to determine extracellular LDH activity. The intracellular LDH activity was estimated after cells lysis with the assay buffer contained in the kit. All samples were incubated with a supplied reaction mixture, resulting in a product where its absorbance was measured at 450 nm using Victor 3 plate reader [35 (link)].
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