Extraction and Quantification of Analytes

Accurately weigh 5 g powder to be tested and placed in a 50 mL conical flask, 10 mL of 70% methanol was added, the plug was covered, its quality was recorded, and the water temperature was controlled at 40°C. The ultrasonic was 45 min and stirred every 15 min with ultrasonic power of 50 kHz. Let it sit overnight, ultrasound again for 45 min, cool it at room temperature, weigh the weight again, use 70% methanol to make up the weight lost, mix well, transfer it to 15 mL centrifuge tube, centrifuged at 4,000 rpm for 10 min, the supernatant was filtered by 0.22 μm microporous membrane to obtain the test solution.