After treatment, the worms were transferred into EP tubes. The worms were washed with M9 buffer by low-speed centrifugation, fixed in pre-cooled 1% paraformaldehyde for 10 min, again washed with M9 buffer, and permeabilized with pre-cooled acetone at -20℃ for 2 min. After washing with M9 buffer, the worms were incubated with 100 nmol/L rhodamine phalloidin at room temperature for 2 h in the dark and then washed with M9 buffer. Droplets containing the C. elegans were loaded onto glass slides and photographed under a confocal laser microscope as previously described [25, (link)26] (link) .