Organotypic Skin Reconstructs: Epithelial Differentiation

Organotypic reconstructs were grown as previously described [18 (link), 20 (link)] with the exception that each culture was rinsed in 1XPBS and incubated with Epidermalization 3 medium lacking serum for two additional days prior to harvesting. The following treatments were added to the organotypic cultures at the time of epithelial seeding and renewed with every media change: 5 ng/ml recombinant human TGFβ1, 10 ng/ml Activin A, 100 ng/ml Follistatin-288 (all from R&D Systems) or 1 μM A83-01 (Tocris).

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Taylor C., Loomans H.A., Le Bras G.F., Koumangoye R.B., Romero-Morales A.I., Quast L.L., Zaika A.I., El-Rifai W., Andl T, & Andl C.D. (2015). Activin a signaling regulates cell invasion and proliferation in esophageal adenocarcinoma. Oncotarget, 6(33), 34228-34244.

Publication 2015

Activin A Follistatin-288 A83-01

A83 01 Activin a Follistatin Human Organotypic cultures Serum Tgfβ1

Corresponding Organization : Vanderbilt University Medical Center

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Variable analysis

independent variables

5 ng/ml recombinant human TGFβ1
10 ng/ml Activin A
100 ng/ml Follistatin-288
1 μM A83-01

dependent variables

Organotypic culture characteristics

control variables

Each culture was rinsed in 1XPBS and incubated with Epidermalization 3 medium lacking serum for two additional days prior to harvesting

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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